precipitation assay meaning in English

沉淀分析,沉淀反应测定

By yeast two - hybrid assay , aes was found to interact with gp130 intracellular region through its conserved q domain . results from the yeast two - hybrid assay , gluthione s - transferase fusion protein pull - down assay and immuno - co - precipitation assay indicated that the q domain of tle1 is capable of binding gp130 intracellular domain , and the intracellular membrane proximal region of gp130 containing conserved boxl and box2 motifs seemed essential for this interaction . to investigate the consequence of this interaction , tle1 - gfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with gp130 expression vector
在通过酵母双杂交分析确定aes通过q结构域与sp130分子胞浆区结合的基础上，为确定tle1分子是否也能通过保守的q结构域与gp130分子胞浆区结合，我们通过pcr扩增编码gp130胞浆区与tleq分子不同结构域的cdna ，构建了含有这些不同结构域的酵母双杂交载体，通过酵母双杂交分析证实： tle1分子通过其氨基端的q结构域与gp130分子胞浆区近膜段结合。